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Image Search Results
Journal: Journal of biotechnology
Article Title: Next generation 1536-well oligonucleotide synthesizer with on-the-fly dispense
doi: 10.1016/j.jbiotec.2013.11.027
Figure Lengend Snippet: NG-1536-AMOS (top left): (I) reagent assembly, (II) 16-tip valve head, and (III) titer plate holder with drain block underneath. The drive system below the plate holder (III D) consists of X, Y and Z axes (motor-driven tracks); (I) reagent and gas supply assembly (tubing and wires omitted for clarity): (A) wash manifolds for purging reagent lines after a completed run, (B) central tubing manifold for argon gas supply to (C) the reagent bottles, (D) 3-way valves for switching from wash (acetonitrile) to reagent and vice versa, and (E) valve assembly and dispense head. Each phosphoramidite position is designed to hold Applied Biosystems 2 g bottles, and oxidizer, activator, and capping reagents are in 200 ml bottles (Applied Biosystems); (II) valve assembly and dispense head: (A) tubing from reagent bottles to dedicated valves, (B) valve block outer shell, (C) valve-nozzle junction, and (D) nozzle head (4 × 4 configuration); (III) top view of titer plate assembly: (A) reagent purge and prime manifold for dispensing reagents, (B) 1536-well titer plate (C) top plate that sits above the titer plate assembly for sealing in argon gas (a strip of silicone rubber and Teflon are bracketed in around the titer plate for maximum seal between the top and bottom plates during synthesis to minimize gas leakage), (D) waste basin for collecting spent reagents during drain steps of each synthesis cycle, (E) bottom plate, (F) center hole where the 16-nozzle head is positioned above the titer plate during synthesis, (G) argon gas jet inlets, (H and J) gas line-in, (I) reagent-to-waste out (vacuum), and (K) prime station purge-to-waste. Note: arrows indicate direction of gas/vacuum/waste flow through the system.
Article Snippet: Titer plate and solid support Custom titer plates with 1536-well
Techniques: Blocking Assay, Stripping Membranes